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In some experiments the bacterial surface was desialylated with 13· In the earlier set of experiments that utilized neuraminidase, we noted that, following neuraminidase treatment, there were typically fewer cells in the dish, indicating that cells had lost cell-cell ...

Dendritic cells (DCs) play an essential role in immunity against bacteria by phagocytosis and by eliciting adaptive immune responses. The phagocytosis of different pathogenic E. coli isolates was also enhanced by sialidase, which suggests that modifications on MDDC sialic ...

To assay for oxidoreductase activity, the purified recombinant proteins were incubated in 100-μl reactions at 37 °C overnight with 1 mg/ml 2,7-anhydro-Neu5Ac or Neu5Ac in 20 mm sodium phosphate buffer, pH 7.5, in the presence 500 μm NADH. The ...

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It was beforehand believed that influenza pandemics occurred at 10- to 14-12 months intervals, nevertheless it has been over 30 years since H3N2 viruses appeared. Viruses have been first remoted in Hong Kong in July 1968. Widespread illness with ...

Background was washed out with deionized water and gel was scanned with the default instrument settings for analysis (Lexmark CX727 scanner). Inhibition of glycolipid synthesis was carried out as described previously (36) with some modifications. Resialylation was carried out ...

The data from these assays were complemented by a glycan array analysis which determined that AAV1 efficiently binds to NeuAcα2-3GalNAcβ1-4GlcNAc as well as glycoproteins containing NeuAcα2-3/α2-6Galβ1-4GlcNAc N-linked glycans. Sialic acids are typically found as terminal monosaccharides of glycans carried ...

This was followed by the addition of neuraminidase solution or buffer control to each well and continued resistance measurement 4 (or more) h. AAV6 relies more on sialic acid or sialic acid-containing glycoproteins than AAV1 for cell entry and/or ...

They symbolize mid-factors in industrial reactions in multi-step processes, and in some cases can pace up chemical production by avoiding the need for earlier phases. If you have any kind of concerns regarding wherever as well as how to ...

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